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Identification of differentially expressed genes induced in pancreatic islet neogenesis
Author(s) -
Rafaeloff Ronit,
Qin Xiao F.,
Barlow Scott W.,
Rosenberg Lawrence,
Vinik Aaron I.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01457-8
Subject(s) - neogenesis , biology , gene , hamster , complementary dna , pancreas , islet , microbiology and biotechnology , genetics , endocrinology , insulin
Cellophane wrapping of the hamster pancreas induces islet neogenesis. We have used the mRNA differential display technique to select for genes expressed during islet neogenesis but not in control pancreata. Ten candidate clones have been identified. Upon sequencing, 6 clones showed a high degree of homology to known genes, 1 showed some, and 3 showed no homology to genes of known sequence. Thus, mRNA differential display is a useful technique to identify genes induced during islet neogenesis, and in combination with screening hamster pancreatic cDNA libraries for full length clones, will enhance the likelihood of capturing the participants in this process.