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Molecular characterization of a cDNA encoding functional human deoxyhypusine synthase and chromosomal mapping of the corresponding gene locus
Author(s) -
Bevec Dorian,
Kappel Barbara,
Jaksche Herbert,
Csonga Robert,
Hauber Joachim,
Klier Hannelore,
Steinkasserer Alexander
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01456-x
Subject(s) - biology , biochemistry , complementary dna , microbiology and biotechnology , gene , cdna library , genetics
Deoxyhypusine synthase is essentially required for the post‐translational formation of hypusine, a modification of a specific lysine residue in eukaryotic initiation factor 5A, which appears to be pivotal for cell proliferation. From a human peripheral blood mononuclear cells cDNA library we isolated two independent sequences encoding biologically active deoxyhypusine synthase. DNA sequence analysis revealed a 369 amino acid protein with a molecular mass of 41.055 kDa. This recombinant deoxyhypusine synthase showed significant catalytic activity in synthesis of deoxyhypusine after in vitro transcription and translation as well as upon expression in Escherichia coli . Using a panel of somatic rodent‐human cell hybrids we localized the deoxyhupusine synthase gene to human chromosome 19.