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Schizosaccharomyces pombe possesses an unusual and a conventional hexokinase: biochemical and molecular characterization of both hexokinases
Author(s) -
Petit Thomas,
Blázquez Miguel A.,
Gancedo Carlos
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01451-9
Subject(s) - hexokinase , glucokinase , schizosaccharomyces pombe , glycerol kinase , biochemistry , fructose , glycerol , enzyme , biology , dehydrogenase , isozyme , schizosaccharomyces , glycolysis , chemistry , gene , saccharomyces cerevisiae
Two hexokinases were characterized in Schizosaccharomyces pombe : hexokinase 1, with a low phosphorylation coefficient on glucose ( K m 8.5 mM) and hexokinase 2, a kinetically conventional hexokinase. Genes hxk1 + and hxk2 + encoding these enzymes were cloned and sequenced. Disruption of hxk1 + had no effect on growth but disruption of hxk2 + doubled the generation time in glucose. Spores carrying the double disruption hxk1 + hxk2 + did not grow on glucose or fructose after one week. Expression of hxk1 + increased strongly during growth in fructose or glycerol. Expression of hxk2 + was highest during growth in glycerol. A NADP‐dependent glucose dehydrogenase was detected, but not a glucokinase.