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Modification of the targeting presequence of the bovine cytochrome P ‐450scc precursor lifting tissue‐specific restrictions on its mitochondrial import
Author(s) -
Novikova L.A.,
Savel'ev A.S.,
Zvyagil'skaya R.A.,
Luzikov V.N.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01443-8
Subject(s) - biochemistry , cytochrome , mitochondrion , biology , chemistry , enzyme
It has been found that a recombinant cytochrome P ‐450scc precursor supplemented with an extra MRGSH 6 GIR sequence at the NH 2 ‐terminus (6His‐pP450scc) is imported into isolated rat liver and heart mitochondria as well as into yeast mitochondria. The import is coupled with proteolytic processing of the precursor resulting in the mature size form of cytochrome P ‐450scc. Modification of the targeting presequence responsible for its increased positive charge is supposed to lift the previously suggested tissue‐specific restrictions on the pP450scc import into mitochondria.