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Cloning and tissue expression of two cDNAs encoding the peroxisomal 2‐enoyl‐CoA hydratase/3‐hydroxyacyl‐CoA dehydrogenase in the guinea pig liver
Author(s) -
Caira Françoise,
Cherkaoui-Malki Mustapha,
Hoefler Gerald,
Latruffe Norbert
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01425-x
Subject(s) - peroxisome , cloning (programming) , guinea pig , microbiology and biotechnology , biochemistry , biology , chemistry , gene , genetics , computer science , programming language
The 2‐enoyl‐CoA hydratase/3‐hydroxyacyl‐CoA dehydrogenase (HD) is the second enzyme of the peroxisomal β‐oxidation pathway. In human and rat, only one HD mRNA has been so far detected in the liver. This paper reports for the first time in a mammal species, the guinea pig, the cloning and sequencing of two cDNAs encoding an HD. The 3,274 nucleotide‐cDNA is a strictly identical but longer copy of the 2,494 nucleotide‐form. A 2,178 by‐open reading frame encodes a protein of 726 amino acids ( M r 79.3 kDa) with the peroxisomal‐targeting signal (tripeptide SKL) at the carboxyterminus. Northern blot analysis of HD mRNA identified three mRNAs of respective sizes 3.5, 2.6 and 1.6 kb in the guinea pig liver and kidneys.