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Adenosine evokes potassium currents by protein kinase C activated via a novel signaling pathway in superior colliculus neurons
Author(s) -
Nishizaki Tomoyuki,
Ikeuchi Youji
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01399-7
Subject(s) - phospholipase c , protein kinase c , pertussis toxin , microbiology and biotechnology , adenosine , intracellular , g protein , gq alpha subunit , potassium channel , protein kinase a , chemistry , biophysics , signal transduction , phospholipase , biology , biochemistry , kinase , enzyme
Adenosine evoked whole‐cell potassium currents and enhanced intracellular free Ca 2+ concentration ([Ca 2+ ]i) in superior colliculus neurons through a P 2Y purinoceptor linked to a pertussis toxin‐insensitive G‐protein, possibly Gq‐protein, which is involved in a protein kinase C(PKC) activation pathway. The [Ca 2+ ]i increase was inhibited by a phospholipase C(PLC) inhibitor, whereas the evoked currents were not affected by a PLC inhibitor or a phospholipase A 2 (PLA 2 ) inhibitor. Adenosine elicited single channel currents via PKC activation in cell‐attached patches and furthermore, those currents with conductances of the same slope were induced even in excised patches, suggesting that PKC can be activated only by cell membrane factors without intracellular components. These results thus indicate that the P 2Y purinoceptor‐coupled potassium channel is regulated via a novel PKC activation pathway independent of PLC or PLA 2 .