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A novel yeast protein showing specific association with the cyclin‐dependent kinase 5
Author(s) -
Huang Q.-Q.,
Lee K.-Y.,
Wang J.H.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01394-6
Subject(s) - open reading frame , activator (genetics) , fusion protein , yeast , biology , gene , saccharomyces cerevisiae , microbiology and biotechnology , biochemistry , complementary dna , peptide sequence , cyclin dependent kinase 5 , homology (biology) , protein kinase a , kinase , recombinant dna , mitogen activated protein kinase kinase
The present study describes a significant amino acid sequence homology between neuronal Cdk5 activator (nck5a) and an open reading frame of an unknown gene on the yeast S. cerevisiae chromosome III. A cDNA encoding a 25 kDa fragment of this yeast protein, the region containing homologous sequence to nck5a was cloned and expresed in E. coli as a glutathine‐ S ‐transferase fusion protein (GST p25Y). GST‐p25Y was found to block the in vitro activation of Cdk5 by nck5a and to affinity precipitate Cdk5 from bovine brain extract. The observations indicate that the yeast protein is capable of specific and high affinity association with Cdk5.