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Heterologous complementation of peroxisome function in yeast: the saccharomyces cerevisiae PAS3 gene restores peroxisome biogenesis in a Hansenula polymorpha per9 disruption mutant
Author(s) -
Jan A.K.W. Kiel,
Ineke KeizerGunnink,
Thomas Krause,
Masaharu Komori,
Marten Veenhuis
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01385-7
Subject(s) - complementation , saccharomyces cerevisiae , peroxisome , heterologous , biogenesis , yeast , biology , mutant , heterologous expression , biochemistry , gene , recombinant dna
PER genes are essential for the biogenesis of peroxisomes in the yeast Hansenula polymorpha . Here we describe the functional complementation of a H. polymorpha per9 disruption strain (Δ per9 ) by a heterologous gene. The Saccharomyces cerevisiae PaAbstractp, a homologue of Per9p, restored peroxisome biogenesis and peroxisomal protein import in the Δ per9 mutant, allowing it to grow again on methanol as sole carbon and energy source. This result shows that heterologous complementation of peroxisome function in yeast is indeed feasible and furthermore suggests that H. polymorpha Δper9 may be the candidate of choice to attempt the isolation of Per9p homologues from higher eukaryotes by functional complementation.