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Molecular cloning of the defense factor in the albumen gland of the sea hare Aplysia kurodai
Author(s) -
Nobuhiko Takamatsu,
Tadayoshi Shiba,
Koji Muramoto,
Hisao Kamiya
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01375-x
Subject(s) - complementary dna , signal peptide , biology , peptide sequence , aplysia , glycoprotein , biochemistry , amino acid , cloning (programming) , cdna library , glycosylation , snail , molecular cloning , microbiology and biotechnology , gene , ecology , computer science , programming language , evolutionary biology
Aplysianin‐A, an antibacterial glycoprotein in the albumen gland of the sea hare Aplysia kurodai , inhibited the growth of both Gram‐positive and Gram‐negative bacteria. Aplysianin‐A cDNA clones were isolated from an albumen gland cDNA library. Sequence analysis reveals that aplysianin‐A is produced as a precursor protein of 556 amino acid residues with a signal peptide of 19 amino acid residues and contains 6 potential N ‐glycosylation sites. Aplysianin‐A mRNA was expressed tissue‐specifically in the albumen gland. Homology search reveals that aplysianin‐A has a 50% overall amino acid sequence homology to achacin, an antibacterial glycoprotein of the giant African snail Achatina fulica .