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Cooperative phenomena in the photocycle of D96N mutant bacteriorhodopsin
Author(s) -
Alexey N. Radionov,
Andrey D. Kaulen
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01367-9
Subject(s) - bacteriorhodopsin , chemistry , acceleration , kinetics , light intensity , azide , photochemistry , biophysics , mutant , optics , physics , biochemistry , biology , membrane , organic chemistry , classical mechanics , quantum mechanics , gene
The M intermediate decay in the photocycle of D96N mutant bacteriorhodopsin does not depend on the light intensity of the exciting flash. Cooperative phenomena in the photocycle are revealed after addition of azide causing acceleration of the M decay and making it kinetically well separated from the N decay. Increase in the light intensity induces slight deceleration of the M decay and significant acceleration of the N decay. The data obtained directly confirm our recent model [Komrakov and Kaulen (1995) Biophys. Chem. 56, 113–119], according to which appearance of the M slow intermediate in the photocycle of the wild type bR at high light intensity is due to destabilization of the N intermediate leading to the acceleration of the N→M and N→bR reactions.