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Phospholipid‐synthesizing enzymes in Golgi membranes of the yeast, Saccharomyces cerevisiae
Author(s) -
Leber Andrea,
Hrastnik Claudia,
Daum Günther
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01361-x
Subject(s) - golgi apparatus , phosphatidylethanolamine , phospholipid , biochemistry , endoplasmic reticulum , phosphatidylinositol , saccharomyces cerevisiae , membrane , phosphatidylcholine , golgi membrane , yeast , chemistry , biology , microbiology and biotechnology , signal transduction
Golgi membranes of the yeast, Saccharomyces cerevisiae , were isolated by a method similar to the procedure described by Cleves et al. [Cell 64 (1991) 789–800]. Marker proteins of the Golgi, such as Kex2 protease and GDPase, are highly enriched in these preparations. The phospholipid and ergosterol content of Golgi membranes is low. Phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol are the major phospholipids of this compartment. The amount of phosphatidylserine in the Golgi is significantly higher than in yeast bulk membranes. Inositol‐containing sphingolipids, especially inositolphosphorylceramide, are highly enriched in Golgi membranes. Two phospholipid‐synthesizing enzymes, namely phosphatidylinositol synthase and sn ‐1,2‐diacylglycerol cholinephosphotransferase, are detected in the Golgi at a specific activity which exceeds that of the endoplasmic reticulum.