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Co‐selection of cognate antibody‐antigen pairs by selectively‐infective phages
Author(s) -
Krebber Claus,
Spada Stefania,
Desplancq Dominique,
Plückthun Andreas
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01348-2
Subject(s) - antigen , virology , antibody , phage display , periplasmic space , biology , bacteriophage , phagemid , infectivity , microbiology and biotechnology , genetics , gene , escherichia coli , virus
We have developed a chloramphenicol resistant derivative of fd phage with which cognate pairs of antibodies and antigens can be selected. The phage genome encodes a fusion of single‐chain antibody to the C‐terminal domain of gIIIp, rendering the phage non‐infective. The antigen fused to the N‐terminal domains of gIIIp is encoded in the same phage genome. Antigen and antibody fusion interact with each other in the periplasm of the phage‐producing cell, restoring infectivity. This system has a very low background and will allow simultaneous randomisation of antibody and antigen.