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Immunological evidence that HMG‐CoA reductase kinase‐A is the cauliflower homologue of the RKIN1 subfamily of plant protein kinases
Author(s) -
Ball Kathryn L.,
Barker Jacqueline,
Halford Nigel G.,
Hardie D.Grahame
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01343-1
Subject(s) - kinase , biology , biochemistry , protein subunit , protein kinase a , microbiology and biotechnology , cyclin dependent kinase 7 , reductase , saccharomyces cerevisiae , enzyme , mitogen activated protein kinase kinase , gene
Three different antibodies against the RKIN1 and BKIN12 gene products from rye and barley recognized the 58 kDa subunit of HMG‐CoA reductase kinase‐A (HRK‐A) from Brassica oleracea on Western blots. HRK‐A was also detected by an antipeptide antibody in enzyme‐linked immunoassays, and this was competed by the peptide antigen. HRK‐A was not recognized by antibodies against plant, mammalian and Saccharomyces cereviseae relatives of RKIN1, i.e. wheat PKABA1, rat AMP‐activated protein kinase and S. cerevisiae Snf1p. RKIN1/HMG‐CoA reductase kinase‐A are now among the first protein kinases in plants to be well characterized at both the molecular and biochemical levels.