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Oxidized low‐density lipoprotein induces the production of superoxide by neutrophils
Author(s) -
Ryouta Maeba,
Akiko Maruyama,
Osamu Tarutani,
Nobuo Ueta,
Hiroyuki Shimasaki
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01336-9
Subject(s) - superoxide , chemistry , phagocytosis , oxidative phosphorylation , biochemistry , cytochalasin , lipid peroxidation , reactive oxygen species , respiratory burst , cytochalasin b , in vitro , biology , immunology , oxidative stress , enzyme , cell , cytoskeleton
Exposure of guinea pig peritoneal neutrophils to ox‐LDL led to the production of superoxide, which was measured by the formation of superoxide‐dependent chemiluminescence. The cells exposed to unoxidized LDL, e.g. native LDL, acetyl‐LDL, and self‐aggregates of LDL showed no production of superoxide. The superoxide production was correlated with the levels of oxidative modification of LDL and reached a maximum between 10 and 30 min during incubation, but preincubating the cells with cytochalasin B decreased the superoxide production. These findings indicate that neutrophils rapidly take up ox‐LDL by phagocytosis and generate superoxide which may cause superoxide‐mediated lipid peroxidation in vivo.