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Isolation of CF 0 CF 1 from Chlamydomonas reinhardtii cw15 and the N‐terminal amino acid sequences of the CF 0 CF 1 subunits
Author(s) -
Fiedler Heike R.,
Schmid Roland,
Leu Stefan,
Shavit Noun,
Strotmann Heinrich
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01332-6
Subject(s) - chlamydomonas reinhardtii , chlamydomonas , amino acid , protein subunit , chloroplast , biology , biochemistry , peptide sequence , cyanobacteria , gel electrophoresis , strain (injury) , microbiology and biotechnology , gene , genetics , bacteria , anatomy , mutant
CF 0 CF 1 was isolated from chloroplasts of the cell wall‐deficient Chlamydomonas reinhardtii strain cw15. The subunit pattern was analyzed by SDS‐gel electrophoresis and the N‐terminal amino acid sequences of all nine subunits were determined by microsequencing. The amino acid sequences of subunits α, β, γ and ϵ match with those derived from the corresponding Chlamydomonas DNA sequences. In variance with the previously assumed N‐terminus of β; however, it was found that the first 11 amino acids are lacking. The subunits δ, I, II, III and IV were identified by comparison with known sequences of homologous polypeptides of higher plant chloroplasts and cyanobacteria, respectively.