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Quantification of DNA damage and repair in amino acid auxotrophs and UV‐sensitive mutants of Aspergillus nidulans using an ELISA
Author(s) -
Donnelly E.,
Barnett Y.A.,
McCullough W.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01316-4
Subject(s) - mutagen , auxotrophy , dna damage , dna repair , aspergillus nidulans , mutant , biology , dna , methionine , biochemistry , arginine , amino acid , mutagenesis , nucleotide excision repair , microbiology and biotechnology , chemistry , gene
An ELISA used to investigate DNA repair in mammalian cells has been adapted to investigate mutagen‐induced DNA damage and repair in protoplasts of Aspergillus nidulans . The assay shows a reduced rate of repair of DNA damage in methionine and arginine auxotrophs ( meth G and arg B), which were shown previously to be hypersensitive to UV radiation and chemical mutagens. The assay also showed a considerably reduced ability to repair mutagen‐induced damage in the uv ‐sensitive mutants uvs B and uvs H. The increased sensitivity of amino acids auxotrophs to mutagens is, therefore, correlated with a reduced capacity to repair mutagen‐induced DNA damage.