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Cloning and characterisation of the rabbit 5‐HT 1Dα and 5‐HT 1Dβ receptors
Author(s) -
Harwood Gail,
Lockyer Michael,
Giles Heather,
Fairweather Neil
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01308-3
Subject(s) - receptor , peptide sequence , cloning (programming) , ketanserin , amino acid , microbiology and biotechnology , biology , molecular cloning , biochemistry , gene , protein primary structure , 5 ht receptor , 5 ht2 receptor , serotonin , computer science , programming language
The genes encoding the rabbit 5HT 1Dα and 5HT 1Dβ receptors have been cloned. The deduced amino acid sequence of these receptors shows 91–92% amino acid sequence identity with their human homologues, and similar high sequence identity with homologues from other species. The receptors were transiently expressed in COS‐7 cells and exhibit a pharmacological profile closely resembling their human homologues, including a higher affinity of ketanserin for the 5‐HT 1Dα subtype. However, sumatriptan had a lower affinity for both the rabbit receptors compared to their human counterparts. This may be accounted for by differences between the primary amino acid sequences of these species homologues.