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A rainbow trout SRY‐type gene expressed in pituitary glands
Author(s) -
Ito Michihiko,
Ishikawa Masako,
Suzuki Shin,
Takamatsu Nobuhiko,
Shiba Tadayoshi
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01307-5
Subject(s) - rainbow trout , biology , trout , complementary dna , open reading frame , testis determining factor , microbiology and biotechnology , gene , northern blot , messenger rna , high mobility group , hmg box , recombinant dna , gene expression , endocrinology , peptide sequence , transcription factor , genetics , fishery , dna binding protein , fish <actinopterygii> , y chromosome
A Sox (SRY‐type HMG box) gene, designated SoxP1, was isolated from a cDNA library made from pituitaries of immature rainbow trout. Sequence analysis indicated that the cDNA had an open reading frame encoding 467 amino acid residues containing a DNA binding motif, known as the high mobility group (HMG) box. Northern blot analysis showed trout SoxP1 mRNA was detected in pituitaries and gonadal tissues, but not in liver, spleen, and heart. In pituitaries, trout SoxP1 mRNA was more abundant in immature fish than in mature fish. Gel shift retardation analysis indicated that the recombinant HMG box protein of SoxP1 produced in E. coli had a DNA binding property for an AACAAT or AACAAAG sequence. These findings suggest that the trout SoxP1 protein may play certain roles in growth or maturation in pituitaries as a transcription factor.