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Formation of 8‐nitroguanine in DNA treated with peroxynitrite in vitro and its rapid removal from DNA by depurination
Author(s) -
Yermilov Vladimir,
Rubio Julieta,
Ohshima Hiroshi
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01281-6
Subject(s) - peroxynitrite , depurination , chemistry , nitric oxide , in vitro , dna , biochemistry , dna damage , superoxide , reactive nitrogen species , peroxynitrous acid , antioxidant , organic chemistry , enzyme
Peroxynitrite is a strong oxidant formed by reaction of nitric oxide with superoxide in inflamed tissues. We have demonstrated that 8‐nitroguanine is formed dose‐dependently in calf thymus DNA incubated with low concentrations of peroxynitrite in vitro. 8‐Nitroguanine in acid‐hydrolyzed DNA was chemically reduced into 8‐aminoguanine, which was analyzed using high performance liquid chromatography with electrochemical detection. Only peroxynitrite, but not nitrite, tetranitromethane nor NO‐releasing compounds, formed 8‐nitroguanine. Antioxidants and desferrioxamine inhibited the reaction. 8‐Nitroguanine was depurinated from DNA incubated at pH 7.4, 37°C ( ). Peroxynitrite did not increase 8‐oxoguanine levels in DNA.