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TNF‐α inhibits glucose‐induced insulin secretion in a pancreatic β‐cell line (INS‐1)
Author(s) -
Zhang Shaoying,
Kim Ki-Han
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01272-9
Subject(s) - endocrinology , medicine , insulin , secretion , chemistry , cell culture , biology , genetics
Recent studies suggest that TNF‐α affects various biochemical and physiological processes which may be linked to the etiology of non‐insulin‐dependent diabetes mellitus (NIDDM). For example, TNF‐α interferes with the signaling of the insulin receptor and the metabolism of glucose transporters. The possibility that TNF‐α might directly reduce glucose‐stimulated insulin secretion in pancreatic β‐cells was examined by using an established pancreatic β‐cell line (INS‐1). TNF‐α did not affect glucose‐induced acute insulin secretion (30 min). However, over a longer time period (24 h), TNF‐α decreased glucose‐induced insulin secretion without affecting the total amount of insulin in the cell. In the presence of TNF‐α levels of 0, 10, 100 and 1000 U/ml, the respective 20 mM glucose‐induced insulin secretion was 1.736 ± 0.166, 1.750 ± 0.302, 1.550 ± 0.200, and 1.400 ± 0.112 mU/ml per 3 × 10 5 cells in 24 h.