Evidence for direct involvement of the sarcoplasmic reticulum Ca 2+ ‐ATPase in a passive monovalent cation (K + /Na + ) exchange | Zendy

de Jesus Florence | Zendy; Cuillel Martine | Zendy; Dupont Yves | Zendy

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Evidence for direct involvement of the sarcoplasmic reticulum Ca 2+ ‐ATPase in a passive monovalent cation (K + /Na + ) exchange

Author(s) -

de Jesus Florence,

Cuillel Martine,

Dupont Yves

Publication year - 1995

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/0014-5793(95)01267-1

Subject(s) - endoplasmic reticulum , chemistry , ion exchange , atpase , ion , enzyme , biochemistry , organic chemistry

A specific inhibitor of SERCA‐pumps, thapsigargin (TG) was used to demonstrate the direct involvement of the SR Ca 2+ ‐ATPase in passive K + /Na + exchange. The K + ‐potential variations across vesicle membranes were measured in the absence of ATP with a fluorescent probe: 3,3′‐dipropylthiodicarbocyanine iodide. Addition of EGTA dissipates the K + ‐potential whereas the presence of TG abolishes this effect. Our data prove that the Ca 2+ ‐ATPase translocates monovalent cations at a rate similar to the E 2 →E 1 conformational change.

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