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The phorbol 12‐myristate 13‐ acetate (PMA)‐induced oxidative burst in rat peritoneal neutrophils is increased by a 0.1 mT (60 Hz) magnetic field
Author(s) -
Roy Sashwati,
Noda Yasuko,
Eckert Valorie,
Traber Maret G.,
Mori Akitane,
Liburdy Robert,
Packer Lester
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01266-x
Subject(s) - respiratory burst , phorbol , chemistry , microbiology and biotechnology , oxidative phosphorylation , protein kinase c , biochemistry , biology , signal transduction
Magnetic fields (MF) may affect biological systems by increasing free radical concentrations. To test this, we have investigated whether low frequency (60 Hz) low intensity (0.1 mT) MF can modulate the phorbol 12‐myristate 13‐ acetate (PMA) induced respiratory burst in primed rat peritoneal neutrophils, followed in real time using the dye 2′,7′‐dichlorofluorescin (DCFH), which reacts with free radical‐derived oxidants such as H 2 O 2 (which is formed from the dismutation of superoxide) to become 2′,7′‐dichlorofluorecein (DCF), a highly fluorescent compound. In the presence of the MF, a 12.4% increase in the fluorescence signal was observed in PMA‐stimulated neutrophils ( n = 5, P < 0.02, 18 pairs of measurements). We believe this represents the first experimental observation of MF influencing events involving free radical species generated during signal transduction in living cells.