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Influence of extracellular matrices on ganglioside pattern of two hepatoma cell lines with different adhesive properties
Author(s) -
Fritsch Martin,
Geilen Christoph C.,
Heidrich Claudia,
Reutter Werner
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01265-8
Subject(s) - cell culture , ganglioside , fibronectin , bovine serum albumin , cell adhesion , extracellular matrix , adhesion , chemistry , cell , extracellular , biochemistry , microbiology and biotechnology , biology , genetics , organic chemistry
A cell culture model was developed to investigate the involvement of gangliosides in cell‐matrix adhesion. Two cell lines with different adhesive properties derived from solid Morris hepatoma 7777 were established. Cultured in horse serum‐containing medium, the adhesive cell line (MH 7777 A ) adheres and spreads on uncoated culture dishes, whereas the revertant cell line (MH 7777 A>N ) does not adhere and grows in suspension. The adhesiveness of both cell lines is dependent on the coating protein used (none, bovine serum albumin, fibronectin or collagen I) and the horse serum concentration in the culture medium. Both cell lines, although of the same origin, differed in their ganglioside composition. The most abundant ganglioside of both MH 777 A and MH 7777 A>N cell lines was fucosyl‐G M1 , 0.78 and 0.72 μg per mg cellular protein, respectively. The G M3 and G D1a content of MH 7777 A>N cells was significantly higher than that of MH 7777 A cells. Furthermore, a matrix‐dependency of the ganglioside pattern of both cell lines was demonstrated.