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Steroid hormones and temperature induce changes of binding parameters of their receptors in intact cells
Author(s) -
Rossini Gian Paolo,
Malaguti Claudia
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01264-6
Subject(s) - cycloheximide , receptor , incubation , glucocorticoid receptor , endocrinology , medicine , steroid hormone , hormone , estrogen receptor , estrogen , ligand (biochemistry) , pelp 1 , steroid , glucocorticoid , biology , chemistry , biochemistry , protein biosynthesis , cancer , breast cancer
When MCF‐7 cells were treated with 17β‐estradiol, dexamethasone, or promegestone at 37°C, the K d of receptors for their cognate ligands was found to decrease as compared to that measured at 2°C. Cell incubation with hormone at 37°C did not affect the B max of glucocorticoid and progesterone receptors, but caused a 40% increase of that of estrogen receptor. This increase required the presence of ligand, was insensitive to cycloheximide, and was completed within 10 min of cell incubation at physiological temperature. We conclude that an early step of estrogen action is the recruitment of pre‐existing receptor molecules through activation of their binding capacity.