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Expression of cDNA for a bark lectin of Robinia in transgenic tobacco plants
Author(s) -
Tazaki Kiyoshi,
Yoshida Kazumasa,
Shinohara Kenji,
Koshiba Tomokazu,
Yamamoto Naoki
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01254-0
Subject(s) - lectin , complementary dna , robinia , western blot , microbiology and biotechnology , biology , bark (sound) , biochemistry , nicotiana tabacum , molecular mass , botany , gene , ecology , enzyme
A cDNA encoding a bark lectin of Robinia pseudoacacia was introduced into tobacco plants. The expression of the lectin cDNA under control of the 35S promoter was confirmed by Western blot analysis and a hemagglutination assay of extracts of transgenic plants. Western blot analysis revealed that the subunit of the lectin from tobacco had a molecular mass of 29 kDa. The sequence of nine amino acids from the N‐terminus of the lectin from transgenic tobacco plants was identical to that of the bark lectin from Robinia , indicating that the lectin had been processed correctly at its N‐terminus in tobacco. The molecular mass of the purified native lectin produced by tobacco plants was estimated to be 112 kDa by gel filtration on a column of Superdex 200. It is suggested that the lectin subunits assembled to form tetramers in transgenic tobacco plants.