Premium
Expression of an archaeal chaperonin in E. coli : formation of homo‐ (α, β) and hetero‐oligomeric (α + β) thermosome complexes
Author(s) -
Waldmann Thomas,
Nitsch Michael,
Klumpp Martin,
Baumeister Wolfgang
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01248-8
Subject(s) - thermoplasma acidophilum , chaperonin , escherichia coli , protein subunit , cytoplasm , gene , recombinant dna , biology , biochemistry , archaea , chemistry , microbiology and biotechnology , enzyme
Co‐expression of the two genes encoding the α‐ and β‐subunit of the Thermoplasma acidophilum thermosome in Escherichia coli yielded fully assembled hetero‐oligomeric complexes (α + β). Surprisingly, also separate expression of both genes resulted in formation of hexadecameric complexes (α, β) in the bacterial cytoplasm. On electron micrographs these complexes were indistinguishable from each other and from the native thermosome. The recombinant a‐complex as well as the native thermosome could be reconstituted in vitro from their dissociated subunits in the presence of Mg‐ATP.