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Mono‐, di‐ and trimeric binding of a bis‐netropsin to DNA
Author(s) -
Zasedatelev A.S.,
Borodulin V.B.,
Grokhovsky S.L.,
Nikitin A.M.,
Salmanova D.V.,
Zhuze A.L.,
Gursky G.V.,
Shafer R.H.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01230-c
Subject(s) - netropsin , polynucleotide , chemistry , dna , molecule , ligand (biochemistry) , circular dichroism , crystallography , stereochemistry , binding site , base pair , biochemistry , minor groove , receptor , organic chemistry
An unusual 3:1 stoichiometry for complex formation between an elongated bis‐netropsin compound and its binding site on DNA has been observed. Circular dichroism measurements distinguish two types of complexes formed between this bisnetropsin and poly[d(A‐T)] · poly[d(A‐T)]. The first type is characterized by a 1:1 saturating ratio of bound molecules per ten base pairs. Formation of the second type results from the cooperative binding of two additional bis‐netropsin molecules to the first type of complex. In contrast to these results observed for binding to the alternating polynucleotide, only the 1:1 type of complex is formed when this ligand binds to the homopolymer poly(dA) · poly(dT).