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Identification of the pore forming element of Semliki Forest virus spikes
Author(s) -
Spyr Christian A.,
Käsermann Fabian,
Kempf Christoph
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01197-m
Subject(s) - semliki forest virus , propidium iodide , chemistry , proteolysis , biophysics , iodide , biochemistry , biology , rna , inorganic chemistry , apoptosis , programmed cell death , gene , enzyme
Pore formation at mildly acidic pH by SFV spike proteins was investigated using isolated and modified virions. Modification of the virions was performed by limited proteolysis in presence of octylglucoside and resulted in the formation of E1 particles and spikeless particles, respectively. Pore formation was detected by measuring the influx of propidium iodide into the viral particles. The results obtained clearly showed that the presence of E1 alone is sufficient to promote pore formation at mildly acidic pH. Thus E1 represents the pore forming element of the viral spike proteins.