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Ligands of purinergic receptors stimulate electrogenic H + ‐transport of neutrophils
Author(s) -
Suszták Katalin,
Káldi Krisztina,
Kapus András,
Ligeti Erzsébet
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01181-d
Subject(s) - purinergic receptor , chemistry , receptor , nucleotide , arachidonic acid , biochemistry , phospholipase a2 , intracellular , agonist , phospholipase c , microbiology and biotechnology , enzyme , biology , gene
The possible role of ATP, acting as a ligand on cell surface receptor was investigated in the activation of the electrogenic H + ‐transporting pathway of porcine neutrophil granulocytes. (1) ATP brought about 2.1‐fold increase in the rate of H + ‐efflux. (2) The order of potency of different nucleotides suggests, that ATP acts on P 2 type purinoceptor. (3) The effect of the nucleotides was prevented by inhibition of phospholipase A 2 . (4) Inhibition of the metabolism of arachidonic acid (AA) via the cyclooxygenase pathway had no effect, whereas inhibition of the lipoxygenase pathway significantly enhanced H + ‐release. This is the first report about activation of the H + ‐transporter by physiological stimulator acting on the cell surface.