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Inverse deuterium kinetic isotope effect for peroxidation in human low‐density lipoprotein (LDL): a simple test for tocopherol‐mediated peroxidation of LDL lipids
Author(s) -
Witting Paul K.,
Bowry Vincent W.,
Stocker Roland
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01172-b
Subject(s) - chemistry , lipid peroxidation , vitamin e , low density lipoprotein , radical , antioxidant , lipoprotein , ubiquinol , tocopherol , biochemistry , deuterium , cholesterol , mitochondrion , coenzyme q – cytochrome c reductase , cytochrome c , physics , quantum mechanics
α‐Tocopherol (α‐TOH) can act as a pro‐ or anti‐oxidant for isolated ubiquinol‐10‐free human low density lipoprotein (LDL). We demonstrate that α‐TOH is a more potent pro‐oxidant than other forms of vitamin E for LDL peroxidation initiated by mild fluxes of aqueous peroxyl radicals and low concentrations of Cu 2+ . A simple deuterium exchange test shows that α‐TOH switches from pro‐ to anti‐oxidant at Cu 2+ : LDL ratios 2.5. The results suggest that this test may be useful to distinguish ‘inhibited’ perioxidation of emulsion lipids propagated via the lipid peroxyl radical from that mediated via the antioxidant radical.