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rap 1 p21 regulates the interaction of ras p21 with RGL, a new effector protein of ras p21
Author(s) -
Ikeda Masahiro,
Koyama Shinya,
Okazaki Michiko,
Dohi Kiyohiko,
Kikuchi Akira
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01169-f
Subject(s) - rap1 , effector , protein kinase a , kinase , microbiology and biotechnology , biology , anti apoptotic ras signalling cascade , phosphorylation , gtp' , chemistry , signal transduction , biochemistry , mapk/erk pathway , enzyme
We have recently found that ralGDS family members (RGL and ralGDS) are putative effector proteins of ras p21. rap 1 p21 is a small GTP‐binding protein which has the same amino acid sequence as the effector loop of ras p21. We examined the effect of rap 1 p21 on the interaction of ras p21 with RGL. The GTP‐bound form of rap 1 p21 interacted with RGL as well as did ras p21. rap 1 p21 inhibited the interaction of ras p21 with RGL. RGL was phosphorylated by cyclic AMP‐dependent protein kinase (protein kinase A). Phosphorylation of RGL did not affect its binding to ras p21 and rap 1 p21 under the conditions that phosphorylation of Raf‐1 reduced its affinity for ras p21. These results demonstrate that rap 1 p21 but not protein kinase A regulates the interaction of ras p21 with RGL and suggest that rap 1 p21 and protein kinase A may cooperate to distinguish the signal of ras p21 to RGL from that to Raf‐1.