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Conversion of short‐chain ceramides to short‐chain ceramide GM3 in B16 melanoma cells
Author(s) -
Komori Hironobu,
Ito Makoto
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01137-4
Subject(s) - ceramide , sphingosine , chain (unit) , biochemistry , chemistry , sphingolipid , glycosphingolipid , microbiology and biotechnology , biology , apoptosis , physics , receptor , astronomy
We report that short‐chain ceramide (Cer), C 2 ‐ and C 6 ‐Cer, were immediately glycosylated and finally converted to short‐chain Cer GM3 in B16 melanoma cells. By addition of either C 2 ‐ or C 6 ‐Cer to a cell culture of B16 melanoma in the presence of [ 14 C]Gal, the radiolabeled precursor, was incorporated into each of two novel glycosphingolipids (GSLs) within 30 min along with synthesis of normal GSLs. These novel GSLs were identified as C 2 ‐, C 6 ‐Cer cerebrosides and C 2 ‐, C 6 ‐Cer GM3, respectively. In comparison with C 2 ‐Cer, C 6 ‐Cer was found to be much more efficiently converted to the GSLs, whereas no glycosylated sphingosine was detectable when it was added in place of short‐chain Cer.