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Calbindin 28kDa and calbindin 30kDa (calretinin) are substantially localised in the particulate fraction of rat brain
Author(s) -
Michael J. Hubbard,
Neil McHugh
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01135-2
Subject(s) - calretinin , calbindin , calcium , cytosol , organelle , chemistry , particulates , fraction (chemistry) , biophysics , biochemistry , microbiology and biotechnology , biology , chromatography , enzyme , immunohistochemistry , immunology , organic chemistry
Calbindin 28kDa is implicated in cytosolic calcium transport and calcioprotection functions, principally as a mobile calcium buffer. Using immunoblotting, we have found that 36% of total calbindin 28kDa is in the particulate fraction of rat brain. Particulate calbindin 28kDa was located both within and outside organelles and required detergent for solubilisation. Equivalent observations were made for calbindin 28kDa , 27% of which was insoluble. These findings indicate that a substantial proportion of calbindin does not function as a mobile calcium buffer, and perhaps instead has a calcium signalling role through target ligands in the insoluble cellular fraction.