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Up‐regulation of endothelial nitric oxide synthase expression by cyclic guanosine 3′,5′‐monophosphate
Author(s) -
Ravichandran Lingamanaidu V.,
Johns Roger A.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01134-z
Subject(s) - cyclic guanosine monophosphate , guanosine , endothelial nitric oxide synthase , nitric oxide synthase , chemistry , guanosine monophosphate , nitric oxide , microbiology and biotechnology , biochemistry , biology , nucleotide , gene , organic chemistry , enos
In this study, the role of cyclic GMP, the end product of the NO‐cyclic GMP signalling pathway, in the regulation of ecNOS was investigated. Bovine pulmonary endothelial cells were exposed to 8‐bromo‐cyclic GMP and its effect on NO production, ecNOS protein, and mRNA levels was analyzed. Endothelial cells on exposure to 8‐bromo‐cyclic GMP produced significantly increased amounts of NO, detected as increased cyclic GMP in cocultures with vascular smooth muscle cells both under basal conditions and with agonist stimulation. 8‐Bromo‐cyclic GMP significantly increased the ecNOS protein and mRNA levels as detected on Western and Northern blots respectively. This 8‐bromo‐cyclic GMP mediated increase of NO production, ecNOS protein and mRNA levels suggests that cyclic GMP up‐regulates the expression of ecNOS. Thus, there may be an intercellular feedback mechanism involved at the molecular level in the expression of the NO‐cyclic GMP signalling pathway in blood vessels.