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Helix‐loop‐helix transcription factors regulate Id2 gene promoter activity
Author(s) -
Neuman Külliki,
Nornes Howard O.,
Neuman Toomas
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01128-2
Subject(s) - basic helix loop helix , helix (gastropod) , transcription factor , transcription (linguistics) , gene , microbiology and biotechnology , chemistry , genetics , biology , dna binding protein , ecology , linguistics , philosophy , snail
Id‐like helix‐loop‐helix (HLH) transcription factors are involved in the regulation of proliferation and differentiation of several cell types. We isolated 5′ regulatory region of mouse Id2 gene and demonstrated that it contains several E‐box clusters. These E‐boxes mediate stimulatory effects of basic‐HLH (bHLH) transcription factors ME1, ME2, and NSCL1 on Id2 promoter activity. Co‐expression of Id2 blocks the stimulatory effect of bHLH transcription factors which suggests the presence of feedback loops in Id2 transcriptional regulation. Overexpression of NSCL1 in F9 cells blocks the downregulation of Id2 gene expression during retinoic acid induced differentiation. Our data demonstrate that bHLH transcription factors regulate Id2 gene expression.