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Expression of cytochrome P450 CYP1B1 in breast cancer
Author(s) -
McKay Judith A.,
Melvin William T.,
Ah-See A.K.,
Ewen Stanley W.B.,
Greenlee William F.,
Marcus Craig B.,
Burke M.Danny,
Murray Graeme I.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01126-y
Subject(s) - cyp1b1 , breast cancer , cytochrome p450 , messenger rna , reverse transcriptase , cancer research , biology , reverse transcription polymerase chain reaction , cancer , polymerase chain reaction , gene expression , enzyme , microbiology and biotechnology , gene , biochemistry , genetics
The expression of CYP1B1 has been identified in breast cancer using the reverse transcriptase‐polymerase chain reaction and immunoblotting. CYP1B1 mRNA was expressed in the majority of breast tumours and immunoblotting of breast tumours identified a single protein band of molecular weight 60 kDa corresponding to the predicted molecular weight of human CYP1B1. This is the first study to identify CYP1B1 expression in a tumour where it may represent a previously unknown pathway for the metabolism of oestradiol and chemotherapeutic drugs.