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DNA strand breakage and base modification induced by hydrogen peroxide treatment of human respiratory tract epithelial cells
Author(s) -
Spencer Jeremy P.E.,
Jenner Andrew,
Chimel Ken,
Aruoma Okezie I.,
Cross Carroll E.,
Wu Reen,
Halliwell Barry
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01117-w
Subject(s) - hydrogen peroxide , dna , dna damage , respiratory tract , breakage , chemistry , base pair , biochemistry , microbiology and biotechnology , biophysics , respiratory system , biology , materials science , anatomy , composite material
Treatment of human respiratory tract epithelial cells with H 2 O 2 led to concentration‐dependent DNA strand breakage that was highly‐correlated with multiple chemical modifications of all four DNA bases, suggesting that damage is due to hydroxyl radical, OH . . However, the major base damage occurred to adenine. Hence, conclusions made about the occurrence and the extent of oxidative DNA damage on the basis only of changes in 8‐hydroxyguanine should be approached with caution.