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An ICE‐like protease is a common mediator of apoptosis induced by diverse stimuli in human monocytic THP.1 cells
Author(s) -
Zhu Huijun,
Fearnhead Howard O.,
Cohen Gerald M.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01116-v
Subject(s) - thp1 cell line , mediator , apoptosis , microbiology and biotechnology , protease , biology , chemistry , cell culture , genetics , biochemistry , enzyme
Apoptosis was induced in THP.1 cells, a human monocytic tumour cell line, by diverse stimuli including cycloheximide, thapsigargin, etoposide and staurosporine. Induction of apoptosis by all these stimuli, except etoposide, was enhanced in the presence of the trypsin‐like protease inhibitor, chloromethyl ketone (TLCK). Induction of apoptosis, assessed by morphological, flow cytometric and biochemical criteria, including proteolysis of poly(ADP‐ribose) polymerase and cleavage of DNA to large kilobasepair fragments, was completely abrogated when cells were pretreated with an ICE‐like protease inhibitor, ZValAlaAsp·fluoromethylketone. This suggested that an ICE homologue was a common mediator of apoptosis in THP.1 cells.