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Evidence for specific, high‐affinity binding sites for a proteinaceous elicitor in tobacco plasma membrane
Author(s) -
Wendehenne David,
Binet Marie-Noëlle,
Blein Jean-Pierre,
Ricci Pierre,
Pugin Alain
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01108-q
Subject(s) - elicitor , biochemistry , binding site , chemistry , membrane , biophysics , plasma protein binding , biology , enzyme
Binding of cryptogein, a proteinaceous elicitor, was studied on tobacco plasma membrane. The binding of the [ 125 I]cryptogein was saturable, reversible and specific with an apparent K d of 2 nM. A single class of cryptogein binding sites was found with a sharp optimum pH for binding at about pH 7.0. The high‐affinity correlates with cryptogein concentrations required for biological activity in vivo.