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Involvement of the three inter‐α‐trypsin inhibitor (ITI) heavy chains in each member of the serum ITI family
Author(s) -
Héron Antoine,
Bourguig Jeannette,
Diarra-Mehrpour Maryam,
Dautréaux Brigitte,
Martin Jean-Pierre,
Sesboüé Richard
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01103-l
Subject(s) - chemistry
Partial cDNAs coding for each of the three human inter‐α‐trypsin inhibitor (ITI) heavy chains were expressed in a bacterial plasmid system and rabbits were immunised with the fusion peptides obtained. Despite the strong sequence homology of these chains, the antisera turned out to be highly specific in the analysis of corresponding mRNA translation products or partially digested serum ITI. Besides classical serum ITI members, their use in Western blotting made it possible to evidence an H3‐related ITI form and a low‐amount H1‐related HC/bikunin component. The relative levels of ITI family members was further studied in baboon and foetal calf sera.