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Reconstitution photoactive yellow protein from apoprotein and p ‐coumaric acid derivatives
Author(s) -
Imamoto Yasushi,
Ito Takeshi,
Kataoka Mikio,
Tokunaga Fumio
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01096-w
Subject(s) - chromophore , p coumaric acid , chemistry , coumaric acid , absorption (acoustics) , photochemistry , organic chemistry , stereochemistry , biochemistry , materials science , ferulic acid , composite material , antioxidant
We report reconstitution of photoactive yellow protein (PYP) from apoPYP and p ‐coumaric acid derivatives. The addition of p ‐coumaric acid to the apoPYP sample did not result in the recovery of PYP. In contrast, yellow products were obtained by the addition of p ‐coumaryl thiophenyl ester or p ‐coumaric anhydride to the apoPYP sample, the absorption spectra of which were indistinguishable from the spectrum of intact PYP. Our findings provide strong evidence that PYP has the p ‐coumaryl chromophore. This reconstitution technique opens the way for further biophysical studies of PYP using artificial chromophore analogs.