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Crystallization of threonyl‐tRNA synthetase from Thermus thermophilus and preliminary crystallographic data
Author(s) -
Cura V.,
Kern D.,
Mitschler A.,
Moras D.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01089-w
Subject(s) - thermus thermophilus , orthorhombic crystal system , crystallization , crystallography , ammonium sulfate , transfer rna , escherichia coli , chemistry , crystal structure , solvent , biochemistry , rna , organic chemistry , gene
Threonyl‐tRNA synthetase from Thermus thermophilus (ttTRS) has been overproduced in Escherichia coli , purified and crystallized in solutions containing ammonium sulfate and glycerol. The crystals grew in the orthorhombic space group C222 1 with unit cell dimensions a = 119.5 Å, b = 120.0 Å, c = 317.5 Å. The asymmetric unit is constituted of two monomers and the crystals contain 66% solvent. This paper reports the first crystals of ttTRS and preliminary crystallographic results since the presumed crystals of ttTRS described in a previous paper [1] were crystals of aspartyl‐tRNA synthetase [2].