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Detection of β‐1,2‐mannosyltransferase in Candida albicans cells
Author(s) -
Suzuki Akifumi,
Takata Yuka,
Oshie Asayo,
Tezuka Atsuko,
Shibata Nobuyuki,
Kobayashi Hidemitsu,
Okawa Yoshio,
Suzuki Shigeo
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01061-i
Subject(s) - candida albicans , chemistry , microbiology and biotechnology , biology
A particulate insoluble fraction from Candida albicans J‐1012 (serotype A) strain cells was obtained as the residue after extracting a 105,000 × g pellet of cell homogenate with 1% Triton X‐100. Incubation of this fraction with a mannopentaose, Man β 1 → 2Man α 1 → (2Man α 1 →) 2 2Man (αβMan 5 ), in the presence of GDP‐mannose followed by high performance liquid chromatography showed the formation of a mannohexaose. Analysis of the product by 1 H NMR indicates that αβMan 5 was changed to Man β 1 → 2Man β 1 → 2Man α 1 → (2Man α 1 →) 2 2Man (αβMan 6 ). This β‐1,2‐mannosyltransferase (ManTase) II activity was completely inhibited by Zn 2+ and was not restored by the addition of EDTA. The corresponding enzyme fraction from C. albicans NIH B‐792 (serotype B) strain cells, the mannan of which does not possess both the αβMan 5 and αβMan 6 side chains, also exhibited the same β‐1,2‐ManTase II activity.