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Functional expression of the human CHIP28 water channel in a yeast secretory mutant
Author(s) -
Laizé Vincent,
Rousselet Germain,
Verbavatz Jean-Marc,
Berthonaud Véronique,
Gobin Renée,
Roudier Nathalie,
Abrami Laurence,
Ripoche Pierre,
Tacnet Frédérique
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01060-r
Subject(s) - mutant , saccharomyces cerevisiae , yeast , secretory pathway , secretory protein , vesicle , golgi apparatus , microbiology and biotechnology , biochemistry , chemistry , heterologous expression , heterologous , biology , secretion , membrane , endoplasmic reticulum , gene , recombinant dna
The temperature‐sensitive Saccharomyces cerevisiae mutant strain NY17, deficient in the secretory pathway ( sec6‐4 mutation), is used for the heterologous expression of the human CHIP28 water channel. After a heat‐shock, the protein is present in partially purified post‐golgi secretory vesicles. Immunodetection and water transport studies, directly made on the vesicles, showed that CHIP28 is highly expressed and active in the yeast membranes.