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Molecular cloning and functional characterization of a GABA/betaine transporter from human kidney
Author(s) -
Rasola Andrea,
Galietta Luis J.V.,
Barone Virginia,
Romeo Giovanni,
Bagnasco Serena
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01052-g
Subject(s) - betaine , phloretin , gaba transporter , biochemistry , chemistry , transporter , complementary dna , neurotransmitter transporter , biology , microbiology and biotechnology , gene
The human homologue of the canine GABA/betaine transporter (BGT‐1) was isolated from a kidney inner medulla cDNA library. The coding sequence predicts a 614 amino acids protein with the typical features of neurotransmitter transporter family. The gene maps to chromosome 12p13 and, in addition to kidney, is also expressed in brain, liver, heart, skeletal muscle, and placenta. Functional studies reveal a K m = 20 μ M for GABA transport and a coupling to Na + and Cl − with a stoichiometry 3 Na + :2 Cl − :1 GABA. At 500 μM the GABA transport was inhibited by various compounds with the following potency order: quinidine > verapamil > phloretin > betaine.