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Characterization and functional dissection of the galactin‐1 gene promoter
Author(s) -
Salvatore Paola,
Contursi Cristina,
Benvenuto Giovanna,
Bruni Carmelo Bruno,
Chiariotti Lorenzo
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01032-a
Subject(s) - gene , transfection , microbiology and biotechnology , biology , promoter , transcription (linguistics) , transcription factor , endogeny , chemistry , gene expression , genetics , biochemistry , linguistics , philosophy
The galectin‐1 gene encodes a β‐galactoside‐binding protein whose overexpression is associated with neoplastic transformation and loss of differentiation. Transient transfection assays of a series of deletions constructs (pGAT) showed that the galectin‐1 promoter is highly active in cells both expressing and non‐expressing the endogenous gene, and that the basal activity is determined by sequences encompassing the transcription start site ( ). Both an upstream ( ) and a downstream position‐dependent ( ) cis ‐elements are necessary for efficient transcriptional activity and are able to bind nuclear proteins.