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Modulation of obese gene expression in rat brown and white adipose tissues
Author(s) -
Moinat Madelaine,
Deng Chengjun,
Muzzin Patrick,
Assimacopoulos-Jeannet Françoise,
Seydoux Josiane,
Dulloo Abdul G.,
Giacobino Jean-Paul
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01030-i
Subject(s) - brown adipose tissue , endocrinology , medicine , white adipose tissue , gene expression , adipose tissue , biology , messenger rna , northern blot , beta 3 adrenergic receptor , gene , adrenergic agonist , adrenergic , adrenergic receptor , receptor , biochemistry
The ob gene mRNA expression in rat brown adipose tissue (BAT) and epididymal white adipose tissue (WAT) was measured on Northern blots hybridized with a rat ob gene probe. The level of ob gene mRNA in BAT was about 40% of that in WAT. Fasting (36 h) or semi‐starvation (10 days) decreased the ob gene mRNA level in both tissues by 62–68%, and cold exposure at 6°C (24 h) decreased it in BAT (−84%)_but not in WAT. Acute administration of the β 3 ‐adrenergic agonist Ro 16‐8714 decreased the ob gene mRNA level in BAT (−51%) and WAT (−28%) of lean Zucker rats and only in BAT (−74%) of obese falfa rats. This study demonstrates that, in the rat, the ob gene is not only expressed in WAT but also in BAT, and suggests that in these two tissues, the modulation of the ob gene expression might be more closely associated with known alterations in cell lipid content than with changes in sympathetic activity.