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Shear stress induces expression of CNP gene in human endothelial cells
Author(s) -
Okahara Kazuhiro,
Kambayashi Jun-ichi,
Ohnishi Tadashi,
Fujiwara Yoshiyuki,
Kawasaki Tomio,
Monden Morito
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01027-c
Subject(s) - umbilical vein , shear stress , gene expression , messenger rna , transcription (linguistics) , microbiology and biotechnology , chemistry , gene , biology , materials science , biochemistry , in vitro , linguistics , philosophy , composite material
To elucidate the effect of blood flow on gene transcription of C‐type natriuretic peptide (CNP), human umbilical vein endothelial cells were exposed to shear stress in a cone‐plate viscometer. Expression of CNP mRNA, evaluated by reverse transcription‐polymerase chain reaction, was markedly increased by exposure to shear stress of 24 dyne/cm 2 at 3 h. The CNP mRNA level was maintained until 12 h. Thus, the present study demonstrated for the first time that shear stress induces expression of CNP gene in human endothelial cells.