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In vivo treatment of Heymann's Nephritis using a cytotoxic protein‐toxin conjugate
Author(s) -
Misquith Sandra,
Surolia Avadhesha
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01026-b
Subject(s) - conjugate , antibody , antigen , toxin , in vivo , chemistry , immunology , nephritis , virology , microbiology and biotechnology , biology , biochemistry , mathematical analysis , mathematics
In this study we investigated the possibility of treating Heymann's Nephritis (HN) by destroying antibody producing cells by targetting a toxin, gelonin ‐ conjugated to gp330, the renal brush border antigen. HN was induced in rats by immunizing them with purified gp330. The gelonin‐gp330 conjugate was administered 12 days after the antigenic challenge. Serum was screened for circulating antibodies. Proteinurea was estimated. The gp330‐gelonin conjugate‐treated animals had a circulating antibody titre in the serum much lower than that of diseased (untreated) animals. Proteinurea seen in diseased animals was not observed in treated animals. This work suggests the possibility of using a toxin‐antigen conjugate for immunomodulating antibody mediated autoimmune renal disease.