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Identification of a novel human serpin gene; cloning sequencing and expression of leupin
Author(s) -
Barnes Ruth C.,
Worrall D.Margaret
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)01015-7
Subject(s) - serpin , microbiology and biotechnology , biology , hela , complementary dna , gene , cloning (programming) , genomic dna , peptide sequence , cell culture , genetics , computer science , programming language
A novel serpin gene has been isolated, cloned and sequenced. A PCR amplified fragment of the gene was originally identified from human genomic DNA, and the full‐length cDNA was subsequently isolated from HeLa cells and sequenced. The novel serpin is very high in protein sequence similarity (91.8%) to the squamous cell carcinoma antigen (SCCA), but contains substantial differences in the reactive site loop sequence, including a different amino acid (leucine) in the P 1 position. The gene product, named leupin, is expressed in HeLa cells, SKGIIIa cells and human placenta. The protein has a predicted M r of 44,857 and an isoelectric point of 6.04 which is consistent with the more acidic form of SCCA associated with squamous cell carcinomas.